Example of a Microbiology Unknown Lab Report

 

unknown bacteria lab report

Oct 29,  · View Lab Report - unknown bacterium lab report from BIO at College of Charleston. Biology General Microbiology HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY G97%(). Aug 22,  · UNKNOWN LAB REPORT. Unknown Number and Alternative #4 Rebecca Blackmon. April 30th, General Microbiology I: BIO Spring INTRODUCTION: The way bacteria function and flourish is important, especially for people entering the Healthcare field, because it allows people to have a better understanding on how to work with or. Dec 03,  · UNKNOWN LAB REPORT. Unknown number Clare Zilch. December 3, The two microorganisms were identified; one was a Gram-positive bacteria, the other was a Gram-negative bacteria. A series of biochemical tests were performed based on the results of the Gram stains.


Unknown Lab Report | Growth Medium | Bacteria


In this paper I will discuss the processes of unknown bacteria lab report I came to find my two unknown bacteria. This will be a vital task to take with me into my profession for many reasons. In the medical field bacteria and infections of different kinds are the core of the practice. These bacteria must be able to be identified in order to treat patients properly, efficiently and safely. Materials and Methods:, unknown bacteria lab report.

The unknown number handed out by the Professor on March 20, contained both a gram positive bacteria and a gram negative bacteria. At this point everything that had been learned in microbiology lab and that had been explained in our lab manual 1 was put into action. The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate was used. The streak method was used to spread the bacteria across the nutrient agar in hopes of isolating a pure culture of one of the bacteria.

In order to do the streak method, an inoculating loop was sterilized with a Bunsen burner and put into the unknown specimen. After removal with bacteria on the loop, the quadrant streak method was used. The streak plate was then incubated at 37 degrees Celsius for 48 hours. Upon returning and observing the streak plate, there was an abundance of green across the plate. There was only one colony that was apparent. After observation, a sample was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate the colonies.

As well as using the quadrant method to further isolate the colonies, a sample was taken from the best colony on the original streak and gram stained, unknown bacteria lab report. The gram stain procedure was performed as directed in the lab manual 1. The gram stain showed a result of red, gram negative rods. To decipher between which biochemical tests to perform, the gram positive and negative tables handed out by the Professor, were referred to.

From previous biochemical tests done in the semester, Pseudomonas aeruginosa was already suspected because of the green pigment of the original streak plate. Upon reviewing the identification tables, the deciding biochemical test was the Casein test which tests for the production of the enzyme casease to break down the milk protein casein, unknown bacteria lab report.

The milk agar was incubated at 37 degrees Celsius for 48 hours. After observation there was a clear positive result, unknown bacteria lab report, which showed the bacteria produced casease. After confirming the gram negative bacteria, the process of isolating the gram positive bacteria began. Returning to the original unknown stocka quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria.

This MSA plate was incubated at 37 degrees Celsius for 48 hours. Upon return and observation, the MSA did not yield a good isolated colony. Professor Snaric advised to do unknown bacteria lab report MSA agar from the original stock, but this time with a sterile swab instead of an inoculating loop. This test was also incubated at 37 degrees Celsius for 48 hours and returned a good isolated colony.

A sample was taken from this colony and transferred to a nutrient broth agar to further isolate it. After incubation, this nutrient agar had great results with many isolated colonies. A sample was taken from the isolated nutrient agar and a gram stain was done as directed by the lab manual 1. The gram stain showed clear purple gram positive cocci.

After getting a good gram stain the identification tables were referred to in order to choose between appropriate biochemical tests. A nitrate test was performed in order to detect if the bacteria was able to reduce nitrate into nitrate or some further reduced form. Then a urea test was performed to check for the production of urease.

All of the biochemical tests performed were explained, in the lab manual provided by Professor unknown bacteria lab report and were practiced earlier in the semester. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test. The first test performed on the gram negative bacteria, was a Casein Test. This test gave a positive result turning a brown color, meaning the gram negative bacteria produced the enzyme casease in order to break down the milk protein casein.

This was the only test necessary to determine the unknown gram negative bacteria in unknown stock The first test performed on the gram positive bacteria was the Nitrate Test which turned red after adding reagents giving a positive result meaning the bacteria reduced nitrate into nitrite or something further.

Following the nitrate test was the Unknown bacteria lab report Test to determine if the bacteria produced urease. This gave a positive result showing a hot pink broth, meaning the bacteria did produce urease. The unknown contained two different specimen of bacteria, one being a gram positive bacteria and one being a gram negative bacteria.

The first unknown in found to be a gram negative unknown bacteria lab report, was identified as Pseudomonas aeruginosa. This identification was reached by only one biochemical test and close observation.

A gram stain was done originally and found red rods identifying the bacteria as gram negative. The streak plate that was originally done, showed a heavy green pigment, which is a main characteristic of Pseudomonas auruginosa. The next test performed was a Casein Test which showed a clear positive result. The only gram negative bacteria that shows a positive result on the Casein Test is Pseudomonas aeruginosatherefore correctly confirming the unknown gram negative bacteria. The second unknown bacteria was identified as a gram positive bacteria with unknown bacteria lab report coccus shape.

This immediately ruled two of the gram positive bacteria Bacillus cerus and Bacillus subtilis. This left three bacteria that the second unknown could be Staphylococcus aureusStaphylococcus epidermidis, or Enterococcus faecalis.

The next test performed was a Nitrate Test which gave a positive result. The two bacteria that give a positive result for this test are Staphylococcus aureus and Staphylococcus epidermidis. The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria unknown bacteria lab report Staphylococcus epidermidis because Staphylococcus aureus gives a negative result. Consultation with the Professor, confirmed the two unknown bacteria correctly as Pseudomonas aeruginosa and Staphylococcus epidermidis.

The only problem that was encountered appeared when trying to isolate the gram positive bacteria. The gram negative bacteria in unknown is a very aggressive bacteria that makes the growth of a gram positive bacteria difficult. Even on an MSA agar, it took a few tries and several isolations to get the gram positive to successfully grow. After successful isolation of both bacteria, unknown bacteria lab report, there were no more issues encountered in identifying either.

Pseudomonas aeruginosa is a gram negative rod shaped bacteria that was first discovered in by a pharmacists named Carle Gessard 2. His study picked up on the unique blue-green pigmentation of P.

This bacteria can catalyze in many environments which makes it very common and found almost everywhere such as soil, water, humans, plants, unknown bacteria lab report, sewage and hospitals.

This bacteria more so affects individuals with compromised immune systems the most common being those with cystic fibrosis, cancer, or AIDS. It builds resistance against a lot of antibiotics and even chemotherapeutic agents, unknown bacteria lab report. Although unknown bacteria lab report cases have been caused by swimming in pools or hot tubs with incorrect levels of chlorine.

To avoid the spread and contamination of P. Selecting the correct antibiotic for this particular bacteria is unknown bacteria lab report important. The methods learned during previous lab exercises for identifying bacteria have been applied to these unknowns.

All procedure performed were done so according to the […]. July 24, at pm. Post Your Comments Click here to cancel unknown bacteria lab report. Popular Topics acls acls class louisville acls louisville advanced cardiac life support aed american heart association articles basic life support bls class louisville bls nursing student cancer cpr cpr aed cpr certification cpr class cpr class columbus cpr class louisville cpr louisville cpr nursing clinicals dental disease heart disease hypertension medical condition pals pals class louisville pals louisville stroke.

To determine if the enzyme casease was produced to break down the milk enzyme casein. To determine if the bacteria reduced nitrogen to some further reduced form such as nitrite. Postive, the bacteria reduced the nitrate into nitrite or some further form of nitrogen.

 

How to Write Microbiology Unknown Lab Report | Example Paper

 

unknown bacteria lab report

 

not necessary for this type of paper, since this is lab report for the identification of an unknown bacterium and the methods are explained in detail in the lab manual. If there is a procedure that the instructor added or made changes to, or the student used another procedure not in the course lab manual, then it should be written out and. Jan 03,  · Microbiology Unknown Lab Report | Staphylococcus aureus The first procedure performed was an isolation of the unknown bacteria with the goal of obtaining a pure culture. This was conducted by streaking the unknown out on a Trypticase Soy Agar (TSA) plate using the T streak method described in the manual. Lab Manual for General. MICROBIOLOGY UNKNOWN LAB REPORT. Unknown Number Jennifer Mills. April 30, Microbiology, BIO Spring/ INTRODUCTION In the past, it has been vital to distinguish the identities of microorganisms in the world.